FGF2 Protein (AA 135-288)

Catalog No. ABIN7454253

Recombinant FGF2 protein expressed in Escherichia coli (E. coli).

Quick Overview for FGF2 Protein (AA 135-288) (ABIN7454253)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 135-288

Purpose: Human FGF basic (154aa) Protein

Sequence: Ala135-Ser288

Characteristics: Recombinant Human FGF basic (154aa) Protein is expressed from E.coli without tag.It contains Ala135-Ser288.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FGF basic (154aa) at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: FGF basic

Background: FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60 % amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.

Molecular Weight: 17.12 kDa same as Tris-Bis PAGE result.

UniProt: P09038-4

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins